American Airlines has suspended its daily New York to Birmingham service.
The US carrier started the service in 2015 but has confirmed it will suspend it from January 2017.
A spokesman for American Airlines said: "As the world's largest airline, American constantly evaluates our network to ensure we are maximizing our fleet and profitability while matching customer demand.
"Many factors influence network planning decisions; route performance is monitored over time to ensure that American is better positioning itself for long-term success against global competition."
Birmingham Airport said it was disappointed by the decision. "We appreciate that transatlantic air services are particularly competitive, however we are optimistic that another carrier will fill this gap shortly.
"There remains a strong demand from both the Midlands and North American market wanting to access these important regions and cities,” the airport said.